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X ̲ ֲ Ҳ ϲҲ ò (², 8 11 ² 2014 .) ² 2014 MINISTRY OF EDUCATION AND SCIENCE OF UKRAINE ...

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MINISTRY OF EDUCATION AND SCIENCE OF UKRAINE

IVAN FRANKO NATIONAL UNIVERSITY OF LVIV

X INTERNATIONAL SCIENTIFIC CONFERENCE

FOR STUDENTS AND PHD STUDENTS

YOUTH AND PROGRESS OF

BIOLOGY BOOK OF ABSTRACTS

(LVIV, 8 11 APRIL 2014) LVIV 2014 581.1:577 㳿: X ̳ (. , 8 11 2014 .). , 2014. 310 .

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: . . , . . , . . , . . , . . ˺, . . , . . , . . , . . , . . , . . , . . , . . , . . ³, . . , . . , . . , . . , . . ϳ : . . . , . . . , . . . , . . . , . . . , . . . , . . . , . . . , . . . , . . . Youth and Progress of Biology: Book of Abstracts of X International Scientific Conference for Students and PhD Students (Lviv, 8 11 April 2014). Lviv, 2014. 310 p.

Book of abstracts contains the results of scientific work of students and PhD students from Ukraine and other countries. The authors are responsible for the trustworthiness of scientific results and for the text quality.

For scientists, PhD students and students, which work in the field of biology and biomedicine.

The Organizers of the Conference are grateful to the Rectors Office of Ivan Franko National University of Lviv, the Ukrainian-American beneficial foundation Seibr-Svitlo Editorial board: I. Khamar, O. Reshetylo, I. Shevchuk, O. Kusnezh, V. Lesnik, I. Dykyy, M. Mekich, I. Bilska, M. Lupak, Kh. Bohoslavets, A. Halushka, M. Romaniv, Kh. Skrypets, M. Vitushynska, I. Stadnyk, D. Voloshyn, I. Komarnytsky, O. Sachok, N. Pisulinska Scientific committee: ass. prof. Z. Mamchur, prof. G. Antonyak, ass. prof. S. Hnatush, prof. V. Manko, prof. D. Sanagursky, prof. N. Sybirna,prof. L. Tasenkevych, prof. O. Terek, prof. V. Fedorenko, prof. Y. Tsaryk

   

Ͳ ²IJ / PLENARY LECTURES

Karpyn O., Bunyo L., Terek O.

CAREX HIRTA L. PLANTS ADAPTATION TO OIL STRESS

Ivan Franko National University of Lviv 4, rushevskyi St., Lviv, 79005, Ukraine e-mail: olgakarpyn@gmail.com Oil pollution causes increasing concern all around the world. One of the most prospect techniques for restoration of oil contaminated soils is environmentally friendly, cost effective phytoremediation. Long-rootage grasses, known with their high adaptive potential under oil pollution, are often used for phytoremediation of oil contaminated sites. However physiological basis of plant adaptation mechanisms under oil influence remain to be elucidated. A better understanding of these mechanisms will lead to improve remediation traits of resistant plants.

Carex hirta L. plants appear on oil contaminated sites on first stages of their overgrow after oil spills and possess high resistance to oil contamination. The purpose of this work was to study adaptive reactions of Carex hirta plants under crude oil stress. We investigated main growth parameters, amount of primary metabolites, photosynthetic pigments, phytohormones and proantioxidants of 30-days-old Carex hirta plants grown in soil containing 5% crude oil.

Crude oil inhibited growth and accelerated aging of Carex hirta plants. Plant height and leaf size under oil influence reduced. Oil stress also caused reduction of diameter and length of rhizomes. Plagiotropic growth of rhizomes prematurely changed in orthotropic. Oil stress resulted in decreased raw and dry weight of sedge organs. However length of Carex hirta roots under oil pollution increased.

Plant growth depends on phytohormonal balance. Results of our work show that in Carex hirta leaves under oil stress accumulation of abscisic acid and cytokinins occurred. Amount of auxin in sedge leaves under oil stress decreased. Such hormones amount changes can explain inhibition of aboveground part growth. Oil stimulated accumulation of indole-3-acetic acid in rhizomes. Auxin is the main stimulator of root growth, thus its accumulation in rhizomes can serve for support of growth activity under oil stress. Content of abscisic acid increased only in basal part of rhizomes which most actively absorb substances including oil hydrocarbons from the soil.

The influence of oil on content of main plant primary metabolites was studied. It was shown that total amount of carbohydrates, proteins and lipids in Carex hirta plants under oil stress decreased. Slight increase in protein and sugars content was observed only in underground part of the plant. Such inhibition of plants metabolism could be result of photosynthesis suppression under oil influence. Results of our investigation show that oil pollution caused destruction of chlorophyll and disintegration of pigment-protein complexes, especially in older leaves. In young leaves pigment-protein complexes remained more stabile under oil pollution. Oil contamination stimulated accumulation of carothenoids, which play essential role in chlorophyll defense against oxidation in plant leaves.

Results of hydrogen peroxide and lipids peroxidation level determination showed that oxidation processes in sedge plants under crude oil pollution remained on a control level. Crude oil proved to stimulate the accumulation of low molecular weight antioxidants in sedge plants.

High amount of antioxidants could prevent oxidation of cell structures. The most prominent in this process was the role of polyphenols and glutathione. Polyphenols content increased 2 times in rhizomes and 5,6 times in leaves of Carex hirta plants under oil pollution in comparison with control plants. Total antioxidant activity determined by diphenyl picryl hydrasyl scavenging by plant extract also significantly increased, mainly in sedge rhizomes under oil pollution.

-6- 㳿, , 811 2014 .

The results of our investigations suggest that adaptation mechanism of Carex hirta plants to oil stress include stabilization and even inhibition of metabolism as well as growth processes.

Accumulation of antioxidants in sedge rhizomes scavenges oxidation processes and leads to adaptation under oil stress.

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. , 4, , 79005, e-mail: nikola.pirogov@gmail.com

Pirogov M. CLADISTIC ANALYSIS OF THE MORPHOLOGICAL AND MOLECULAR

DATA AND ANALYSIS OF ITS-2 SECONDARY STRUCTURE OF ONE LICHEN

TAXONOMIC GROUP. In modern taxonomy of plants and fungi, there are considered three main species concepts: biological, morphological, and phylogenetic. All the concepts support different methodological approaches and use different data type. Main problem of taxonomy is an unilateral approach of taxonomists while studying a group of organisms. Scientists must conduct comprehensive studies of one dataset to ensure the progress of scientific knowledge in taxonomy.




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-7 Horbal L.1, Kobylyanskyy A.2, Truman A.3, Yushchuk O.1, Marinelli F.2, Ostash B.1, Luzhetskyy A.4, Fedorenko V.1

THE PATHWAY SPECIFIC REGULATORY GENES AS KEYS TO TEICOPLANIN

OVERPRODUCTION IN ACTINOPLANES TEICHOMYCETICUS

Department of Genetics and Biotechnology, Ivan Franko National University of Lviv

   

Helmholtz-Institute for Pharmaceutical Research Saarland, Saarbrucken, Germany e-mail: lihorbal@gmail.com Multidrug resistant bacteria are one of the main and the most dangerous problems all over the world. To treat such serious infections traditional antibiotics, like tetracycline, -lactams are not used. Only drugs of last resort are effective and teicopalnin is one of them. It is much more potent than vancomycin and is used in the fight against many infections, even in children.

Microbiological production the only feasible source of teicoplanin is expensive, largely because of complexity of generation and maintenance of teicoplanin-overproducing strains.

Therefore, there is an acute need in new technologies for teicoplanin overproduction. The only known producer of teicoplanin is Actinoplanes teichomyceticus soil-dwelling bacteria that like Streptomyces possess complex life cycle, however, forms motile spores. This genus of bacteria is much less studied than Streptomyces. However, no doubt that A. teichomyceticus, as well as Streptomyces strains, contains a huge quantity of regulatory genes, that form intricate regulatory networks that precisely govern secondary metabolite biosynthesis in it.

It is reasonable to suppose that regulators of the pathway specific level are, as in Streptomyces, key toggles of certain metabolite production in the genus Actinoplanes. Manipulations with this type of genes in Streptomyces very often lead to overproduction. To this end, we performed search of pathway specific regulators in the teicoplanin (tcp) gene cluster (Sosio et al., 2004; Li et al., 2004). Two putative candidates tcp28 and tcp29, coding for StrR type and LuxR type regulators, respectively, were identified. The inactivation of these genes prevents teicoplanin production, while their overexpression under the control of native promoters 1.5-2 times increases antibiotic yield. These data let us to suppose that Tcp28 and Tcp29 proteins are key switches of teicoplanin production. Given that elevating expression of pathway specific regulators might lead to increase of structural genes transcription and as a result antibiotic overproduction, we substituted native promoters of tcp28 and tcp29 genes with several heterologous. As a consequence replacement of the native promoter with apramycin resistance gene promoter let us to increase teicoplanin production from 120 mg l-1 to on average 4 g l-1. Taking into account obtained data, we conclude that the technology based on manipulation tcp-regulatory genes is a simple, fast, innovative single step way to boost teicoplanin biosynthesis and reduce the production costs.

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̲ ί в . , 4, , 79005, e-mail: lytvvol@gmail.com Lytvyn V., Maksymiv H., Pasternitska I., Kolisnyk Y. INFLUENCE OF SORBIC ACID

ON COMPOSITION OF LUMINAL MICROBIOTA IN LARGE INTESTINE OF RATS. The

-8- 㳿, , 811 2014 .



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